Thermo Scientific™ FastDigest SmaI //Thermo Scientific™ FD0663
Catalog/Part Number:: 10110890
₦47,610.00
Description
The FastDigest SmaI restriction enzyme recognizes CCC^GGG
sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer
(Isoschizomers: TspMI, XmaCI; neoschizomer: XmaI)
Features
- 100%
activity of all FastDigest enzymes in the universal buffer
- 100%
buffer compatibility with downstream applications
- Complete
digestion in 5-15 minutes
- Direct
loading on gels
- No
star activity
- 176
FastDigest enzymes available
Applications
- Molecular
cloning
- Restriction
site mapping
- Genotyping
- Southern
Blot
- Restriction
fragment length polymorphism (RFLP)
- SNP
analysis
Note
The FastDigest Green Buffer offers the same high performance
in DNA digestion and downstream applications as the colorless FastDigest
Buffer. For applications that require product analysis by fluorescence
excitation (e.g. concentration measurements in UV light) the colorless
FastDigest Buffer is recommended.
For methylation sensitivity refer to product specifications.
Technical Specifications
| 10x FastDigest Buffer/FastDigest Green Buffer | |
| Not dam methylation-sensitive, Not dcm methylation-sensitive, CpG methylation-sensitive | |
| FastDigest | |
| 100μL | |
|
|
Sma I |
| 37° C | |
| Yes |
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