R&D Systems™ Substrate Reagent Pack

Description

Warm to room temperature before use. Color Reagent A and Color Reagent B should be mixed together in equal volumes within 15 minutes of use. Protect from light. Add 100μL of the mixture to each well and incubate at room temperature for 20 minutes while protecting from light. Add Stop Solution (Catalog # DY994) and read the microplate.